virtualbox.org

I just installed Virtual Box 6.1.0 and tried using my existing Windows 95 VM and it fails to boots with a 'Windows Protection error'. It worked fine under VB 6.0.14.
I can confirm that VT-x/AMD-V is turned off for the VM under Processor settings, and rechecked the other settings.
Any ideas what else would cause this error?

Try web-searching:

"Windows Protection error" 95 site:forums.virtualbox.org

Several suggestions arise.