I have the same problem:
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Failed to start the virtual machine Vista.
VT-x is not available. (VERR_VMX_NO_VMX).
Unknown error creating VM (VERR_VMX_NO_VMX).
VM Settings > System > Acceleration > Enable VT-x / AMD-V is enabled, but the checkbox is disabled so I can't deselect it. In fact, both options on that tab are blocked. At the moment I can't do anything past creating a VM. Once created, if I start it after changing settings (eg adding a DVD Drive) it'll go straight to the error. If I start it immediately after creation (without changing any of the VM's settings) then I get the first-run wizard. Once that wizard is complete I get the error on the VM.
Not sure why the first-run doesn't come up if you change the VM Settings before the first time you start it (bug maybe?) but either way, as soon as the VM tries to boot I get the error.
Are there any other ways to disable the VT setting? There's nothing in VirtualBox > Preferences to set it globally either
